Cyanine7-COOH has a wide range of applications in life sciences and medicine. The following are specific applications of Cyanine7-COOH in different applications :

 

1. Biofluorescence imaging: Cyanine7-COOH has near-infrared fluorescent properties and can be used for fluorescence imaging in organisms. For example, Cyanine7-COOH is labeled on antibodies for near-infrared fluorescence imaging of protein distribution and localization on cell surfaces.

 

2. Cellular tracing: Cyanine 7-COOH is labeled on cellular tracer dyes for tracking and localizing the migration and localization of specific cells or cell populations in vivo. For example, Cyanine7-COOH is labeled on a cellular tracer dye (e.g., DiI) for use in near-infrared fluorescence imaging to track the metastasis and localization of tumor cells.

 

3. Molecular probes: Cyanine 7-COOH is labeled on molecular probes for the detection and quantification of the presence and concentration changes of specific biomolecules. For example, Cyanine7-COOH is labeled on DNA or RNA probes for near-infrared fluorescence imaging to detect nucleic acid content and localization within cells.

 

4. Fluorescent labeling: Cyanine 7-COOH is labeled on compounds or biomolecules for fluorescent labeling and analysis. For example, Cyanine7-COOH is labeled on drug molecules for near-infrared fluorescence imaging of drug distribution and metabolism.

 

5. Molecular sensors: Cyanine 7-COOH is labeled on molecular sensors for detecting and measuring the presence and concentration changes of specific molecules in organisms. For example, Cyanine7-COOH is labeled on a pH sensor for near-infrared fluorescence imaging to detect changes in intracellular pH.

 

In conclusion, Cyanine7-COOH has a variety of applications in life sciences and medicine, including biofluorescence imaging, cell tracing, molecular probes, fluorescent labeling and molecular sensors. By labeling Cyanine7-COOH on specific molecules or probes, high-resolution observation and quantitative analysis of specific structures and molecules in organisms can be achieved. Due to the near-infrared fluorescence property of Cyanine7-COOH, it can be used for biomedical imaging under the condition of lower background signal to improve the sensitivity and resolution of imaging.