Cyanine7-NHS has a wide range of applications in life sciences and medicine. Below are specific applications and corresponding examples of Cyanine7-NHS in different applications:

 

1. Protein labeling: Cyanine7-NHS can react with the amino group (-NH2) in proteins to label them. This gives Cyanine7-NHS a wide range of applications in protein labeling and analysis. For example, Cyanine7-NHS can be labeled on antibody molecules and used in immunohistochemical analysis to detect the presence and localization of specific proteins.

 

2. Cell tracing: Cyanine7-NHS can be used to track and localize the migration and localization of specific cells or cell populations in vivo. For example, Cyanine7-NHS is labeled on a cellular tracer dye for tracking the metastasis and localization of tumor cells.

 

3. Molecular probes: Cyanine7-NHS can be labeled on molecular probes for the detection and quantification of the presence and concentration changes of specific biomolecules. For example, Cyanine7-NHS is labeled on DNA or RNA probes for the detection of nucleic acid content and localization within cells.

 

4. Fluorescence imaging: The near-infrared fluorescent nature of Cyanine7-NHS makes it an ideal tool for fluorescence imaging in organisms. For example, Cyanine7-NHS is labeled on fluorescent probes and used for NIR fluorescence imaging to study the distribution and localization of specific structures or molecules in organisms.

 

5. Pharmaceutical research and pharmacokinetics: Cyanine7-NHS can be labeled on drug molecules for pharmaceutical research and pharmacokinetic studies. For example, in drug discovery, Cyanine7-NHS can be labeled on drug molecules to study the metabolic pathways of the drug and the distribution of the drug in the organism.

 

In summary, Cyanine7-NHS has a variety of applications in life sciences and medicine, including protein labeling, cell tracing, molecular probing, fluorescence imaging, as well as drug research and pharmacokinetics. By labeling Cyanine7-NHS on specific molecules or probes, high-resolution observation and quantitative analysis of specific structures and molecules in organisms can be achieved. Due to the near-infrared fluorescence property of Cyanine7-NHS, it can be used for biomedical imaging under the condition of lower background signal, improving the sensitivity and resolution of imaging.